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BIO-286 Cell/Tissue Culture Techniques

Students will receive instruction on the principles and basic techniques used in culturing tissues and cells and then will apply those techniques through hands-on laboratory activities. An emphasis will be placed on mammalian cell culture. Topics covered include sterile and aseptic technique, media preparation, cell count and viability cryopreservation, subculturing, and research applications using cell cultures.

Credits

1

Prerequisite

BIO-121

Lecture Contact Hours

0.5

Lab Contact Hours

1

Other Contact Hours

0

Department

  • Science & Technology

Grading Scheme

  • Letter

SUNY Gen Ed Credit

  • No

Semesters Course Will Be Offered

  • Spring

Course Learning Outcomes

  1. Demonstrate an understanding of the concepts of mammalian cell culture
  2. Utilize lab protocols to carry out common mammalian cell culture techniques
  3. Design and present results of a cell-culture-based experiment
  4. Maintain detailed records of lab work
View Course Outline

BIO 286: Cell/Tissue Culture Techniques

Department

Science and Technology

Course Description

Students will receive instruction on the principles and basic techniques used in culturing tissues and cells and then will apply those techniques through hands-on laboratory activities. An emphasis will be placed on mammalian cell culture. Topics covered include sterile and aseptic technique, media preparation, cell count and viability cryopreservation, subculturing, and research applications using cell cultures.

Credit Hours

1

Contact Hours

Lecture0.5
Lab1
Other0

Grading Scheme

Letter

Semester(s) Course Will Be Offered

Spring

Prerequisites

BIO-121

First Year Experience Course

No

Capstone Course

No

Course Learning Outcomes

  1. Demonstrate an understanding of the concepts of mammalian cell culture
  2. Utilize lab protocols to carry out common mammalian cell culture techniques
  3. Design and present results of a cell-culture-based experiment
  4. Maintain detailed records of lab work

Topic Outline

  1. Cell culture equipment and safety
  2. Sterile and aseptic technique
  3. Biology of the culture cell
  4. Contamination
  5. Culture vessels
  6. Cell culture media
  7. Cell differentiation
  8. Primary cultures
  9. Feeding cells
  10. Subculturing cells
  11. Cell counting
  12. Cryopreservation
  13. Cytotoxicity assays
  14. Tissue culture applications