BIO-286 Cell/Tissue Culture Techniques
Students will receive instruction on the principles and basic techniques used in culturing tissues and cells and then will apply those techniques through hands-on laboratory activities. An emphasis will be placed on mammalian cell culture. Topics covered include sterile and aseptic technique, media preparation, cell count and viability cryopreservation, subculturing, and research applications using cell cultures.
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Course Outline
Department
Science and Technology
Course Description
Students will receive instruction on the principles and basic techniques used in culturing tissues and cells and then will apply those techniques through hands-on laboratory activities. An emphasis will be placed on mammalian cell culture. Topics covered include sterile and aseptic technique, media preparation, cell count and viability cryopreservation, subculturing, and research applications using cell cultures.
Credit Hours
1Semester(s) Course Will Be Offered
Spring
First Year Experience Course
No
Course Learning Outcomes
- Demonstrate an understanding of the concepts of mammalian cell culture
- Utilize lab protocols to carry out common mammalian cell culture techniques
- Design and present results of a cell-culture-based experiment
- Maintain detailed records of lab work
Topic Outline
- Cell culture equipment and safety
- Sterile and aseptic technique
- Biology of the culture cell
- Contamination
- Culture vessels
- Cell culture media
- Cell differentiation
- Primary cultures
- Feeding cells
- Subculturing cells
- Cell counting
- Cryopreservation
- Cytotoxicity assays
- Tissue culture applications