BIO-283 Electrophoresis

Students will receive instruction on the principles of polyacrylamide and agarose gel electrophoresis and then will apply those principles in hands-on laboratory activities focused on these two laboratory methods. Students will engage in the design of experiments and then utilize these tools to explore their experimental questions.

Credits

1

Prerequisite

BIO-121

Department

• Science & Technology

Semesters Course Will Be Offered

• Fall

For more detailed course information view the Course Outline

BIO 283: Electrophoresis

Department

Science and Technology

Course Description

Students will receive instruction on the principles of polyacrylamide and agarose gel electrophoresis and then will apply those principles in hands-on laboratory activities focused on these two laboratory methods. Students will engage in the design of experiments and then utilize these tools to explore their experimental questions.

Credit Hours

1

Contact Hours

Lecture	0.5
Lab	1
Other	0

Grading Scheme

Letter

Semester(s) Course Will Be Offered

Fall

Prerequisites

BIO-121

First Year Experience Course

No

Capstone Course

No

Course Learning Outcomes

1. Differentiate between the use of polyacrylamide and agarose gel electrophoresis techniques in the laboratory.
2. Design an experiment based on a testable hypothesis.
3. Communicate experimental results using standard scientific journal formatting

Topic Outline

1. Polyacrylamide Gel Electrophoresis Principles
2. Polyacrylamide Gel Electrophoresis Equipment and Set Up
3. Pipetting and micropipetting
4. Gel Staining & Photography
5. Standard Curves
6. Western Blotting
7. Agarose Gel Electrophoresis Principles
8. Agarose Gel Electrophoresis Equipment and Set Up
9. Restriction Enzymes
10. Southern Blotting
11. Journal Manuscript Drafting, Submission and Peer Review